The video is not a rip-off of Mister Rogers, it was actually sanctioned and requested by PBS Digital Studios, according to the group's YouTube and Twitter accounts.
The video was remixed, with use of the popular "Auto-tuning" feature, by Symphony of Science's John Boswell.
"You can grow ideas, in the garden of your mind," the video sings.
In addition to the show's main star, the video also features delivery man Mr. McFeely, Henrietta Pussycat, Lady Elaine Fairchilde, Prince Tuesday and the Neighborhood Trolley.
PBS says it "immediately wanted to work together" with Boswell after seeing one of his mash-up videos.
"Turns out that he is a huge Mister Rogers Neighborhood fan," PBS said on its YouTube. "And was thrilled at the chance to pay tribute to one of our heroes. Both PBS and the Fred Rogers Company hope you like John's celebration of Fred Rogers' message."
The video is nearing a million views on YouTube after it was uploaded on Thursday.
This is the first in a series of PBS icons remixed, according to PBS.
